RealCycler CORO-G Kits v.5.3 is Real-time PCR Test Kit, using Taqman-like hydrolysis probes technology, in combination with CHIC technology for the design of the internal amplification control. The kits allows the detection of Coronavirus SARS-CoV-2 and Sarbecovirus E gene RNA in clinical samples using realtime PCR.
The rapid spread and alarming increase in the number of coronavirus disease 19 (COVID-19) cases caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in the community is increasing the burden in economy and healthcare system. The rapid and accurate detection of viruses is increasingly vital to control the sources of infection and help patients prevent further disease progression. Since December 2019, after the outbreak of the SARS-CoV-2in Hubei province of China, there has been a considerable challenge among healthcare professionals regarding the use of nucleic acid tests or clinical characteristics of infected patients as the reference standard make a definitive diagnose of COVID-19 patients. For prevention and control of this pandemic, the early diagnosis of COVID-19 is critical .Patients presenting early-onset of symptoms, clinical characteristics cannot alone define the diagnosis of COVID-19.
Along with advancements in medical diagnosis, nucleic acid detection-based approaches have become a rapid and reliable technology for the detection of viruses. The polymerase chain reaction (PCR) method is considered the ‘gold standard’ for detecting viruses among nucleic acid tests. It is characterized by rapid detection, high sensitivity, and specificity. Progenie molecular from Spain recently got approval from the Central Drugs Standard Control Organisation (CDSCO) for precise results of RealCycler CORO-G Real-Time PCR Kits, helping in the diagnosis of India’s corona cases.
RealCycler CORO-G Kits v.5.3 is Real-time PCR Test Kit, using Taqman-like hydrolysis probes technology, in combination with CHIC technology for the design of the internal amplification control.
The kits allows the detection of Coronavirus SARS-CoV-2 and Sarbecovirus E gene RNA in clinical samples using realtime PCR
The system includes an internal control of amplification based on the technology CHIC (Competitive Heterologous Internal Control) to prevent false negatives due to reaction inhibition. In Chic-Out RealCycler kits, CHIC is out of the AmpliMix to verify the nucleic acid extraction.
A Positive Control is included in the ReaCycler, which consists of a DNA artificial construction of Coronavirus, is included in the ReaCycler
RealCycler CORO-G v.5.3 is an in vitro diagnostic kit of reagents which allows the detection of Coronavirus SARS-CoV-2 and Sarbecovirus RNA in clinical samples
RealCycler CORO-G v.5.3 includes the AmpliMix CORO v.5.3 and a CORO Positive Control, which consists of a DNA artificial construction of Coronavirus. All reagents are ready to use without adding or rebuilding any component.
Component | CORO – G Vials | Volume |
AmpliMix CORO v.5.3 (Retrotranscriptase included) | 10 | 1450 μL |
CORO Positive Control | 1 | 480 μL |
a) Nucleic acids purification
RealCycler kits are compatible with
b) According to the below specifications, thermal profile Programme the “CORO” amplification protocol:
Time | Temperature | Cycles | Fluorescence |
10:00 | 45°C | 1 | OFF |
2:00 | 95°C | 1 | OFF |
0:15 0:30 0:30 | 95°C 55°C 72°C | 45 | OFF ON OFF |
C) PCR reaction set-up
D) Adjust the fluorescence threshold
It is necessary to set the fluorescence threshold for each channel according to the indicated values
CliniExperts smoothly got approval for RealCycler CORO-G Real Time PCR Kits of Progenie Molecular.
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